Using MetaboAnalyst

The web interfaces of MetaboAnalyst are designed to be self-explanatory. Most steps are documented on the top of the corresponding pages. In cases of limited space, mouse-over balloon helps are available. The following tutorials are meant to complement the aforementioned information by providing step-by-step instructions for several of the most common tasks.

Please note, due to the rapid evolution and frequent updates of MetaboAnalyst, many screenshot illustrations are outdated. Therefore, we ask that users do not take those steps verbatim. Instead, users should focus on the the analysis concepts and the workflow for metabolomics data analysis using this tool.

Version Title Note Time Stamp
Version 5.0 Overview of MetaboAnalyst 5.0 A brief introduction of history, design concepts and key features 2021-10-15
Raw MS spectral Processing A detailed tutorial for raw spectral analysis 2021-10-15
Functional Analysis A detailed tutorial to guide functional analysis on MS peaks 2021-10-15
Meta Functional Analysis A detailed tutorial to guide meta-analysis on MS peaks 2021-10-15
Joint Pathway Analysis A brief tutorial to introduce joint pathway analysis 2021-10-15
Enrichment Analysis A detailed tutorial to do enrichment analysis 2021-10-15
Network Exploration A tutorial to guide network analysis 2021-10-15
Statistical Analysis[metadata] A tutorial for statistical analysis with metadata 2021-10-15
Video Demo Series A video demo tutorial series 2021-10-15
Version 4.0 Current Protocols In Bioinformatics (v2019) A comprehensive step-by-step tutorial (128 pages & 137 figures) 2019-09-20
Overview of new features in version 4 A detailed screenshot overview of the three new modules introduced 2018-02-14
Biomarker Meta-Analysis Tutorial -- 2018-02-14
MS Peaks to Pathways Tutorial -- 2018-02-14
Network Explorer Tutorial I Step-by-step tutorial to perform knowledge-driven network analysis 2018-02-14
Network Explorer Tutorial II Step-by-step tutorial to perform data-driven network analysis 2020-11-19
Version 3.0 Current Protocols In Bioinformatics (v2016) A comprehensive step-by-step tutorial (91 pages & 88 figures) 2016-09-07
Raw Spectra Processing using R and XCMS Step-by-step tutorial to processing LC-MS spectra for MetaboAnalyst 2014
Version 2.0 Current Protocols In Bioinformatics (v2011) A comprehensive step-by-step tutorial (48 pages & 51 figures) 2011
Metabolomics data analysis IV Presentation on Metabolomics Conference 2012 2012
Nature Protocols on MetaboAnalyst Detailed explanations of major functions of MetaboAnalyst 2011
Metabolomics data analysis III Presentation on Metabolomics Conference 2011 2011
Version 1.0 Metabolomics data analysis II Presentation on Metabolomics Conference 2010 2010
Metabolomics data analysis I Presentation on Metabolomics Conference 2009 2009
Identification of significant features -- 2009
Two-group classification -- 2009
MS spectra processing and annotation -- 2009
Paired (two time-points) analysis -- 2009
Genome Canada Genome Quebec NIH NSERC CRC
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